Pharmacology Practical Essay Example | Topics and Well Written Essays - 500 words

Pharmacology Practical - Essay Example The experiment provided a uniform temperature (37 degrees) for the enzyme activity, whereas in living systems the temperature usually varies within a given range. According to Yun, crude extracts of orlistat also contain an inactive component, which is a possibility in the in vivo action of orlistat that leads to the disparities between experimental values and theoretical values of IC50 (2010). The suggested dose of Orlistat is one 120 mg capsule immediately before, during or up to one hour after, each main meal. From the statement, In general, at therapeutic doses detection of intact Orlistat in plasma is sporadic and concentrations are extremely low ( Orlistat’s systemic absorption is minimal because most of it is largely maintained in the gastrointestinal tract. This is why extremely low plasma concentrations are observed after the intake of the drug (Bryant et al. 2011). Systemic absorption of Orlistat is not necessary for its activity because it provides its therapeutic action in the stomach lumen and ileum. Orlistat binds irreversibly to the amino acid residues serine present in the active sites of gastric and pancreatic lipases through the formation of covalent bonds. This binding inactivates the enzymes making them unavailable for the hydrolysis of dietary fat (triglycerides) into fatty acids and monoglycerides (Orlistat STADAÂ ® 60mg/120 mg 2011). Consequently, a caloric deficit ensues because of failure of absorption of the undigested triglycerides. The caloric deficit has a helpful outcome on the regulation of